On the second day of slave training featuring Queen Anna, human toilet unit 24 undergoes whip punishment. While serving as a human toilet and performing acts of devotion, he endures various forms of discipline including whipping, foot stomping, foot licking, and anal licking. Queen Anna further subjects him to extreme humiliation with golden showers into his mouth, post-urination cleanup with his tongue, eating her vaginal discharge, receiving feces directly into his mouth, horsewhipping, and forced coprophagia.